Abstract

Palm oil is known as one of the important vegetable oils enriched with health beneficial constituents. Despite using hydraulic pressing in palm oil recovery, enzymatic pretreatment prior to pressing was investigated for its effects on the fatty acid profile and micronutrient contents. An enzyme blend of pectinase, cellulase and tannase was used to pretreat the palm mesocarp prior to hydraulic pressing. Enzymatic pretreatment significantly affected the predominant fatty acids contents in the hydraulically pressed palm oil but the overall fatty acid profile (MUFAs > SFAs > PUFAs) remained unchanged compared to the untreated palm oil sample. α-tocopherol and all four forms of tocotrienols (α-, β-, γ- and δ-) were significantly lower in enzymatic treated sample. In contrast, the enzymatic pretreatment markedly influenced the total carotene content with two-fold higher in β-carotene content in the hydraulically pressed palm oil. Additionally, enzymatically treated palm oil showed reduced cholesterol and raised β-sitosterol contents compares to the untreated sample. Overall, enzymatic pretreatment influenced micronutrient contents in hydraulically pressed palm oil without altering its fatty acid profile.

Keywords

References

1. Aksu, M., Incegul, Y., Kiralan, S. S., Kiralan, M. & Ozkan, G. 2020. Cold Pressed Carrot (Daucus carota subsp. sativus) Seed Oil. In: Ramadan, M. F. (Ed.). Cold Pressed Oils. Massachusetts: Academic Press.
2. Almeida, D. T. D., Viana, T. V., Costa, M. M., Silva, C. D. S. & Feitosa, S. 2018. effects of different storage conditions on the oxidative stability of crude and refined palm oil, olein and stearin (Elaeis guineensis). Food Science and Technology, 39(1), 211-217.
3. AOAC International. 2016. Official Method 996.06: Fat (Total, Saturated, and Unsaturated) in Foods. (20th edition). In Official Methods of Analysis of AOAC International. AOAC International.
4. Eitenmiller, R. R. & Lee, J. 2004. Vitamin E: Food Chemistry, Composition, and Analysis. Boca Raton: CRC Press.
5. Gao, Z., Zhu, Y., Jin, J., Jin, Q. & Wang, X. 2023. chemical–physical properties of red palm oils and their application in the manufacture of aerated emulsions with improved whipping capabilities. Foods, 12(21), 3933.
6. Hu, B., Li, Y., Song, J., Li, H., Zhou, Q., Li, C., Zhang, Z., Liu, Y., Liu, A., Zhang, Q. & Liu, S. 2020. Oil extraction from tiger nut (Cyperus esculentus L.) using the combination of microwave-ultrasonic assisted aqueous enzymatic method-design, optimization and quality evaluation. Journal of Chromatography A, 1627, 461380.
7. Kaseke, T., Opara, U. L. & Fawole, O. A. 2021. Effects of enzymatic pretreatment of seeds on the physicochemical properties, bioactive compounds, and antioxidant activity of pomegranate seed oil. Molecules, 26(15), 4575.
8. Loganathan, R., Tarmizi, A. H. A., Vethakkan, S. R. & Teng, K. T. (2020). Retention of carotenes and vitamin e, and physico-chemical changes occurring upon heating red palm olein using deep-fat fryer, microwave oven and conventional oven. Journal of Oleo Science, 69(3), 167-183.
9. Lomenick, B., Shi, H., Huang, J. & Chen, C. 2015. Identification and Characterization of β-sitosterol Target Proteins. Bioorganic & Medicinal Chemistry Letters, 25(21), 4976-4979.
10. Mustafa, A. H., Rashid, S. S., Rahim, M. H. A., Roslan, R., Musa, W. A. M., Sikder, B. H. & Sasi, A. A. 2022. Enzymatic pretreatment of lignocellulosic biomass: An overview. Journal of Chemical Engineering and Industrial Biotechnology, 8(1), 1-7.
11. Okpuzor, J., Okochi, V. I., Ogbunugafor, H. A., Ogbonnia, S., Fagbayi, T. & Obidiegwu, C. 2009. Estimation of cholesterol level in different brands of vegetable oils. Pakistan Journal of Nutrition, 8, 57-62.
12. Orhevba, B. A., Chukwu, O., Osunde, Z. D. & Ogwuagwu, V. 2013. Influence of moisture content on the yield of mechanically expressed neem seed kernel oil. Academic Research International, 4(5), 252-257.
13. Permana, T., Wiradjaja, N., Sutanto, H. & Surya, V. S. 2022. A Review on the Potential of Natural Antioxidant Sources to Improve Oxidative Stability in Edible Oils. Proceedings of the 6th International Conference of Food, Agriculture, and Natural Resource (IC-FANRES 2021). 4–5 August, 2021, Tangerang, Indonesia. pp 117-125.
14. Prommaban, A., Kuanchoom, R., Seepuan, N. & Chaiyana, W. 2021. Evaluation of fatty acid compositions, antioxidant, and pharmacological activities of pumpkin (Cucurbita moschata) seed oil from aqueous enzymatic extraction. Plants, 10(8), 1582.
15. Rabadán, A., Álvarez-Ortí, M., Gómez, R., Alvarruiz, A. & Pardo, J. E. 2017. Optimization of pistachio oil extraction regarding processing parameters of screw and hydraulic presses. LWT-Food Science and Technology, 83, 79-85.
16. Sorokova, N., Didur, V. & Variny, M. 2022. mathematical modeling of heat and mass transfer during moisture–heat treatment of castor beans to improve the quality of vegetable oil. Agriculture, 12(9), 1356.
17. Tan, C. H., Lee, C. J., Tan, S. N., Poon, D. T. S., Chong, C. Y. E. & Pui, L. P. 2021. Red palm oil: A review on processing, health benefits and its application in food. Journal of Oleo Science, 70(9), 1201-1210.
18. Teixeira, C. B., Macedo, G. A., Macedo, J. A., da Silva, L. H. M. & Rodrigues, A. M. D. C. 2013. Simultaneous extraction of oil and antioxidant compounds from oil palm fruit (Elaeis guineensis) by an aqueous enzymatic process. Bioresource Technology, 129, 575-581.
19. Tirgarian, B., Farmani, J. & Milani, J. M. 2019. Enzyme-assisted aqueous extraction of oil and protein hydrolysate from sesame seed. Journal of Food Measurement and Characterization, 13(3), 2118-2129.
20. Trautwein, E. A. & McKay, S. 2020. The role of specific components of a plant-based diet in management of dyslipidemia and the impact on cardiovascular risk. Nutrients, 12(9), 2671.
21. Yazdi, G. A. P., Barzegar, M., Sahari, M. A., & Ahmadi Gavlighi, H. 2019. Optimization of the enzyme‐assisted aqueous extraction of phenolic compounds from pistachio green hull. Food Science and Nutrition, 7(1), 356-366.
22. Zaaboul, F. & Liu, Y. 2022. Vitamin E in foodstuff: Nutritional, analytical, and food technology aspects. Comprehensive Reviews in Food Science and Food Safety, 21(2), 964-998.
23. Zainul, R., Kurniawati, D., Elida, E., Santosa, I., Adha, S. A., Taridala, A.S., Rosmawaty, R., Hidrawati, H., Purbaningsih, Y., Aisyah, D. D. & Dumasari, D. 2024. comparative analysis of virgin coconut oil (VCO) production methods and their impact on nutritional and chemical properties. Journal of Medicinal and Chemical Sciences, 7, 2092-2107.